State: ReviseType of paper: JCR, ScopusThis study explores how phytochemical composition governs th…

State: ReviseType of paper: JCR, ScopusThis study explores how phytochemical composition governs the synthesis and biofunction of green silver nanoparticles (AgNPs) from Zingiberales species. AgNPs from Alpinia calcarata, Elettaria cardamomum, and Heliconia psittacorum were synthesized under optimized green conditions (0.1 mM AgNO₃, 60°C). LC–MS/MS metabolomics revealed that H. psittacorum AgNPs are stabilized by flavonoid glycosides and protoalkaloids, controlling nanoparticle size (~20 nm) and stability. These nanoparticles exhibited pH-responsive release, with higher silver dissolution under acidic conditions (81% vs. 48% at physiological pH). Biological assays showed strong antimicrobial activity (MIC = 6.25–12.5 µg/mL) and selective anticancer effects against MCF-7 cells (IC50 = 32.8 µg/mL), driven by ROS-mediated mechanisms. Overall, this work links plant metabolomics to functional nanomaterial design for sustainable and stimuliresponsive therapeutic applications.IF: 3.2Q: Q3Available position: 4th04133345133@F09149269613